DNA

Part:BBa_J119004:Design

Designed by: Caleb J. Carr   Group: Eckdahl Lab   (2011-07-21)

BsaI-LacZ2-BsmbI


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 1579
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 1579
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 1579
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 1579
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 7


Design Notes

This is NOT a BioBrick part. It is designed to work exclusively with part J119003 (LacZ-1) to report when properly assembled and transcribed. Its purpose is to report when a Hamiltonian Path Problem node has been successfully reached and left.

Source

PCR amplification of the LacZ template.

References